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a standard plate count have 25-250 colony forming units. why is it considered as a limitation...

a standard plate count have 25-250 colony forming units. why is it considered as a limitation of the standard plate count?

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The reason why 25-250 CF( colony forming units) is considered as a limitation because, some kind of bacteria which are put under examination will have an influence on the size of the colonies and also on the number of them that can be developed on the plate.

The supply of food may also be an issue because colonies close to each other may merge and also there is a chance in which the neighbor colonies may also stimulate their growth.

There are these 2 scientists Breed and Dotterrer who did chose their plates which are countable from triplicate plates from each dilution, but the requiring acceptable plates within 20% on average. Even though the CFU count >300 is totally unsatisfied , but the CFU count <400 is good and satisfiable.

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