Question

You have purified protein Q. SDS page shows a single band of 60 kDA. In a gel filtration experiment, protein Q elutes between alchol dehyrogenase (MW = 160 kDa) and betaamylase (MW = 190 kDa). How many subunits are intact in protein Q?

Answer 1

Answer-

According to the given question-

Here we have purified a protein Q with a single band with size of 60 kDa in SDS. When we do gel filtration experiment the protein Q elute between enzyme alcohal dehydrogenase with molecular weight of 160kDa and beta amylase with molecular weight of 190kDa.

Which means that the protein Q have molecular weight mire than 160 and less than 190. Sprotein Q molecular weight will be 180kDa and have 3 subunit because in SDS we have get the single band of 60kDa , so 180 is multiple of 60*3 which means that the molecular weight of protein Q is 180 kDa and having three subunits.