Answer- A-The time required for a bacterial population to double is called generation time.
Explanation why other options are not correct is given below
B- Colony is a visible group of microorganisms which all originate from a single mother cell, hence a colony comprises a clone of bacteria which are genetically similar.
C- Nitrogen is required by organisms mainly to synthesise proteins, nucleic acids and other nitrogen-containing compounds. while some nitrogen-fixing bacteria present in root nodules and soil fix atmospheric nitrogen and produce ammonia.
D- Agar is only used as a substrate to grow bacteria, bacteria cannot digest or degrade agar therefore it just provides a surface to grow bacteria. while other nutrients are added or mixed with agar which serves as a food source for bacteria.
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