Question

Can
you please explain to me the steps of ELISA testing to determine
whether a person is positive or negative for zika virus? I will be
really thankful I just need to know how can ELISA help me to
determine Zika virus in steps*

Answer #1

Now the microplates are coated with anti-human-IgM class antibodies
to bind to the corresponding antibodies of the sample. So after
washing the wells to remove all unbound sample materials, a
Hoarseradish paroxidase labelled Zika virus antigen is added. This
antigen conjugate binds to the captured specific IgM antibodies.
Now in a second washing step unbound conjugate is removed and now
the immune complex is formed by the bound conjugate is visible by
adding Tetramethylbenzidine Substrate, that gives a blue reaction
product. The intesity of this product is similar to the amount of
specific IgM antibodies in the specimen. Now sulphuric acid is
added to stop the reaction. This produces a yellow endpoint
colour.

By this way we can do the ELISA for Zika virus.

can
u please explain to me how PCR can be used to detect Zika virus in
steps?

can you please give me a detailed walk through on how the
solution for d2ydx2=−k2y is y(x)=C1cos(k x)+C2sin(k x). i really
need help to understand this if you could write each step out and
an explanition for each step

Can someone please explain to me why the medulla is smaller
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already decussated to the opposite side and then descend as the
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please be neat and fully explain all steps. will
vote.
can you give me a double integral where its more optimal to
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coord. dr dtheta

Could you please show me the steps on how you did this?
I claim that more than 70% of my students pass my class. You do
not believe me, so you do a records request to the college and find
out that 85 out of 125 of my students passed my class last
semester. Test at the 0.01 level of significance that my proportion
of passing students is not really 70%.
Thank you!

can you please show all the steps thank you...
Prove by induction that 3n < 2n for all
n ≥ ______. (You should figure out what number goes in the
blank.)
I know that the answer is n>= 4, nut I need to write the
steps for induction

A particle moves with rectilinear motion and is
defined by the relation v= t3-4t2+5t-6. Determine:
I. Times when acceleration is zero
II. Distance and acceleration when time is 2 seconds,
given x0= 0
Hi sir i need this solution very urgent please can any
expert or teacher can solve in 20 to 25 minutes can you please help
me out with this its very urgent and i have limited time i shall be
very thankful to you and thumbs up...

it's really important to me to answer my question correctly
I have some components I need to connect them in one schematic,
but I don't know how to connect them togather, is there any
software that I can just chose (place) the components and then it
will connect them automatically (wiring them)?
if yes please write its name.

Can you please explain the Null Hypotheses to me in laymans
terms? I understand it is a term used to say we do not believe
something is not true?

PLEASE DO NOT OMIT STEPS, EXPLAIN IT DETAILED MIND PLEASE, ALSO
WRITE BIG SO THAT I CAN LOOK AT IT AS I CAN CONFUSE THANK YOU VERY
MUCH!
THIS PROBLEM ALMOST ALWAYS RESOLVED ONLY THAT I HAVE PROBLEMS
WITH THE UNITS AT THE END BECAUSE I WAS SEEING THE SOLUTION AND I
SEE THAT TWO LAST CONVERSIONS DO NOT APPEAR TO ME TO BE INECESSARY.
CAN YOU EXPLAIN ME WHY THAT IS DONE?
IN THE IMAGE OF THE PROBLEM THAT...

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