Question

Can you please explain to me the steps of ELISA testing to determine whether a person is positive or negative for zika virus? I will be really thankful I just need to know how can ELISA help me to determine Zika virus in steps*

Answer 1

Ans:- As IgM antibodies are specific to Zika virus in human serum and plasma.
Now the microplates are coated with anti-human-IgM class antibodies to bind to the corresponding antibodies of the sample. So after washing the wells to remove all unbound sample materials, a Hoarseradish paroxidase labelled Zika virus antigen is added. This antigen conjugate binds to the captured specific IgM antibodies. Now in a second washing step unbound conjugate is removed and now the immune complex is formed by the bound conjugate is visible by adding Tetramethylbenzidine Substrate, that gives a blue reaction product. The intesity of this product is similar to the amount of specific IgM antibodies in the specimen. Now sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour.
By this way we can do the ELISA for Zika virus.