Question

1-You have an F- cell that contains resistance to Vancomycin on the chromosome and Erythromycin resistance on the plasmid. The F+ cell contains resistance to Tetracycline on the chromosome and Naladixic acid on the plasmid, in addition to the F plasmid. Assuming you may create your own media, which antibiotic(s) would you include in the media if you are trying to ONLY allow for the growth of the recombinant cell?

1. Which of the following is true about Zones of Inhibition (ZOI) and Minimum Inhibitory Concentrations (MIC)?
   1. A smaller ZOI and smaller MIC corresponds with a more effective antibiotic
   2. A larger ZOI and larger MIC corresponds with a more effective antibiotic
   3. A larger ZOI and smaller MIC corresponds with a more effective antibiotic
   4. Only the ZOI is important when determining antibiotic effectivenes

Answer 1

Ans) c)A larger ZOI and smaller MIC corresponds with a more effective antibiotic

Explanation:

Zone of inhibition (ZOI) is the circular region around antibiotic drop where bacterial growth does not occur so larger the ZoI more will be effectiveness of antibiotic.

MIC (minimum inhibitory concentration) is the minimum conc of a antibiotic which is capable to prevent the growth of bacteria .So lower the value of MIC of a antibiotic means more effectiveness of a antibiotic against bacteria.