Question

Shown below is some of nucleotide sequence (ssRNA) of a portion of the 5’ end of...

Shown below is some of nucleotide sequence (ssRNA) of a portion of the 5’ end of the covid-19 coronavirus genome.

ATATTAGGTT   TTTACCTACC CAGGAAAAGC ..........................

Name the method that would most likely be used to diagnose an infection caused by this virus.

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Answer #1

rRT-PCR test is used to diagnose an infection caused by Covid-19 virus.

We will isolate the cells from the suspected patient and then do rRT- PCR. it stands for real time reverse transcriptase PCR. The primers specific to this sequence is designed. These primers are forward and reverse primer. Now this primer will anneal to give amplification of this region, when the suspected patient has infection. But in case of non infection, there is not found any amplificatikn of this gene. The conserved sequence are taken in this test which is from their capsid coat.

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