Question

Question – Discuss how the 5’ end of eukaryotic mRNAs are processed; include the function of this processing

Question – Discuss protein splicing

Question – Discuss the functions of the exon joining complex

Question – Discuss RNA export from the nucleus including how ran is recycled

Answer 1

Eukaryotic mRNA are processed by 5' clapping, 3' cleavage and polyadenylation and RNA splicing to remove introns. The main steps of mRNA processing includes addition of signaling factors at the end , removing of those sequences which does not code for amino acid . m RNA processing is very important to form a mature RNA to be properly translated into a appropriate protein sequence.

2. Protein splicing is the post translational event which includes the removal of polypeptide segments called intein and binding of sequence extein on both sides through C terminal ligation process.

3. It plays important role in post transcriptional regulation of mRNA. It forms at the joining of two exons during RNA splicing.

4. RNA is export through certain receptors on the nucleus. It includes tRNA , ribosomal RNA , mRNA and micro RNA etc.