Question

Suppose you have mammalian cells in culture. To a dish of cells that have been growing for 5 weeks, you add a mix of matrix metalloproteinases, gelatinase, collagenase and elastase. After 35 hours, you notice a significant increase in proliferation of the cells. Which of the following is the best explanation for what is happening?

l. Widespread necrosis has occurred, causing cells to proliferate.

ll. Cell-cell junctions have been degraded, causing cells to proliferate.

lll. p53 has been activated, causing cells to proliferate.

lV. Non-homologous end-joining has occurred, causing cells to proliferate.

Answer 1

ANSWER - CORRECT OPTION IS B

here the function of gelatinase collagenase and elastase is to degrade the cellular connections and make them to proliferate.

non homologous end joining is not valid here,as these enzymes are nothing to do with genetics

necrosis means cellular death on a large scale,thus this makes that the cells won't proliferate .thus option a is wrong

while p53 activation is something which can be done on genetic level not by the external enzymes.

here , mettaloproeinase,gellatinas,collagenase, elastase,as the name suggest is focused on degrading the compositions of cell cell interactions,thus option B is correct.