Question

Describe how fat digestion by pancreatic lipase was tested and detected

Answer 1

Pancreatic lipase is a digestive enzyme secreted by the pancreas that pass into small intestine and is responsible for digestion of fat and lipids.
test and detection of fat digestion by pancreatic lipase :

Take a test tube and add milk to it. Now add phenolphthalein to it. The solution will turn pink as the solution is alkaline. When we add pancreatic lipase to the solution, the colour changes from pink to colourless. This is because the pancreatic lipase breaks down the fat in milk into fatty acids which decreases the ph of solution and makes it more acidic.
So the fat digestion by pancreatic lipase was tested and detected by changing of colour. The solution turns colourless in presence of pancreatic lipase.