Question

34. Which of the following techniques uses capillary gel electrophoresis as an allele detection to distancing...

34. Which of the following techniques uses capillary gel electrophoresis as an allele detection to distancing between the reference allele and the variant one?

A. Sanger DNA sequencing

B. PCR-RFLP

C. Pyrosequencing

D. Tag Man

Science   Anatomy-And-Physiology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

B. PCR-RFLP

  • The assay for automated PCR-RFLP genotyping is established by Sell and Lugemwa
  • PCR-restriction fragment length polymorphism (RFLP)-based analysis is also termed as cleaved amplified polymorphic sequence is a accepted method for genetic investigation.
  • This method has been applied for the identification of species to species variation
  • The products obtained from PCR are digested by RFLP enzymes and the results visualized using a DNA sequencer.
0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection...
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is the _____ blot. Select one: a. Northern b. Western c. Northeastern d. Southern e. Southwestern 2. DNA sequencing by controlled termination of replication is called the _____ method. Select one: a. Sanger b. E. coli c. Restriction enzymes d. DNA Microarray e. Ligase f. Polymerase Chain Reaction g. Footprint h. Reverse Trancriptase i. Vector j. Expression k. Fluorescent l. cDNA 3. How...
Consider the following scenarios and select which DNA technology is the best to use, options listed...
Consider the following scenarios and select which DNA technology is the best to use, options listed after scenarios. A. You want to sequence several DNA fragments within a rare bird species' genome, but you don't know anything about the species' genome. Select one: Illumina sequencing, Sanger sequencing, Gene cloning OR PCR B. You want to make copies of an STR locus in order to determine the genotype of a patient. Because this is a human STR, the sequence near the...
Which of the following sequences would run the slowest through an agarose gel during gel electrophoresis?...
Which of the following sequences would run the slowest through an agarose gel during gel electrophoresis? a) 5'- AT -3' b) 5’- ATGCTGC -3’ c) 5’- ATGCTGCAGTTA -3' d) All of these sequences would run at the same speed
Which of the following techniques makes use of reverse transcriptase? Group of answer choices Homologous recombination...
Which of the following techniques makes use of reverse transcriptase? Group of answer choices Homologous recombination Northern blotting PCR Automated Sanger sequencing All of the above None of the above. ------------------------------- Restriction enzymes: Group of answer choices A.) Help protect bacteria from foreign DNA.e B) Always generate “sticky ends" with either 3' or 5' overhangs C) Will not cut at a recognition site if the DNA is methylated. D) Both A and C. All of the above. ------------------------------------------------- DNA polymerase...
Which are matched correctly? A. Copy Number Variant - changes in chromosome number between individuals B....
Which are matched correctly? A. Copy Number Variant - changes in chromosome number between individuals B. Allele-specific oligonucleotide - PCR primers used to detect simple sequence repeat alleles C. In/Del - a short oligonucleotide probe that will hybridize to only one allele D. Single Nucleotide polymorphism - alleles that vary based on number of dri-or-tri nucleotide repeats E. DNA polymorphism - a DNA sequence that occurs in two or more variant forms
Which of the following techniques can be used to isolate proteins? a. Salting out b. Size...
Which of the following techniques can be used to isolate proteins? a. Salting out b. Size exclusion chromatography c. Polyacrilamide gel electrophoresis d. All of these e. None of the above
Which of the following best describes Pulsed-field Gel electrophoresis? It is used for surveillance and investigation...
Which of the following best describes Pulsed-field Gel electrophoresis? It is used for surveillance and investigation of foodborne illness outbreaks. It is the result of frequency mapping. It is used only when larger DNA fragments stop the pulsing electric currents. All of the above describe Pulsed-field Gel electrophoresis. The PFGE samples examined by Lanni show similar illnesses clustered in which one of the following states? Oregon Washington Nevada California Which of the following is included in the case definition of...
1. Chain terminators block further DNA synthesis because they are missing which of the following? Group...
1. Chain terminators block further DNA synthesis because they are missing which of the following? Group of answer choices A. 3' OH B. 5' phosphate C. A fluorescent dye 2.The cycle sequencing reaction generates: Group of answer choices A. Fragments of the same size; because the chain terminator integration site is the same in each newly synthesized fragment B. Fragments of the same size; because the chain terminator integration site can be different in each newly synthesized fragment C. Fragments...
6. Which of the following can be manipulated to promote amino acid production in microbes? A....
6. Which of the following can be manipulated to promote amino acid production in microbes? A. Alteration of promotor to increase binding of the repressor. B. Decrease in cell membrane permeability to prevent release of the amino acid. C. Alteration of enzymes in pathways such as the TCA cycle to halt the pathway and “push” the intermediate towards the production of the amino acid. D. A and C. 8. Which of the following is true about Polymerase Chain Reaction (PCR)?...
1. Which of the following is the correct definition of synteny a) The conservation of gene...
1. Which of the following is the correct definition of synteny a) The conservation of gene order within two genomes b) The conservation of gene function within two genomes c) The percentage of nucleotide sequence identity between two genomes d) The percentage of amino acid sequence identity between two genomes 2. Why is open reading frame scanning not applicable when searching for functional RNA genes? a. the codons for functional RNA molecules are not well understood b. introns make the...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT