Question

34. Which of the following techniques uses capillary gel electrophoresis as an allele detection to distancing...

34. Which of the following techniques uses capillary gel electrophoresis as an allele detection to distancing between the reference allele and the variant one?

A. Sanger DNA sequencing

B. PCR-RFLP

C. Pyrosequencing

D. Tag Man

Science   Anatomy-And-Physiology   
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Answer #1

B. PCR-RFLP

  • The assay for automated PCR-RFLP genotyping is established by Sell and Lugemwa
  • PCR-restriction fragment length polymorphism (RFLP)-based analysis is also termed as cleaved amplified polymorphic sequence is a accepted method for genetic investigation.
  • This method has been applied for the identification of species to species variation
  • The products obtained from PCR are digested by RFLP enzymes and the results visualized using a DNA sequencer.
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