Question

In a cell with severe biotin deficiency, glucose could best be biosynthesized with heavy supplementation of...

In a cell with severe biotin deficiency, glucose could best be biosynthesized with heavy supplementation of which of the following ?

A. Aspartate and lactate

B. fatty acids and glucose-6 phosphate

C. Glutamate and glycerol

D. Alanine and glycerol

Homework Answers

Answer #1

C. Glutamate and glycerol

The biotin is the known water soluble vitamin that act as coenzyme for five carboxylases. In absensce of biotin,non -carbohydrate precurssor of the glucose undergoes conversion and forms pyruvate, process is known as gluconeogenesis(occurs in liver).The lactate,glycerol and amino acids are the non carbohydrate presurssor. The aminoacid glutamate and alanine derive from the muscle protein and lactate derives from the muscles and glycerol is derived from the triglycerides from fat cells. In the absence of biotin, L-glutamate production is prevented and growth of E.coli was reduced. The L-alanine production occurs in absence of the biotin which has been observed in biotin deficient micrococcus sodonensis. Therefore, the Glutamate and glycerol is sufficient to biosynthesize the glucose in absence of biotin.

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