Question

If you sequence an organism's complete genome for the first time and start comparing it to sequences already in the public database, one category will be sequences people have found in related organisms but don't know what the function is in any of them.Explain two other categories.

Answer 1

First we should understand what the genome contains how the genome function.

And locating the gene and their control regions by computer analysis and experimentation.

Here are few techniques:-

• Gene location by sequence inspection
• Experimental techniques for gene location

-> hybridization tests can be determine if a fragment contains transcribed sequences

• Determining the functions of individual genes.
• Assigning gene function by experimental analysis

-> functional analysis by gene inactivation.

-> individual genes can be inactivated by homologous recombination.