Question

The pH can affect the activity of an enzyme-controlled reaction by (Select all that apply)

Check All That Apply

• affecting hydrogen bonding

• changing the shape of the enzymes active site.changing the shape of the enzymes active site.

• altering the interaction between the substrate and the enzyme active site.altering the interaction between the substrate and the enzyme active site.

• changing the ionic state of either the enzyme or the substrate.changing the ionic state of either the enzyme or the substrate.

• altering the allosteric site.

Answer 1

Answer -All of the above

affecting hydrogen bonding.

changing the shape of the enzymes active site

altering the interaction between the substrate and the enzyme active site

changing the ionic state of either the enzyme or the substrate

altering the allosteric site.

pH affects the intermolecular bonds i.e hydrogen bonds (which maintains the tertiary structure and active site ).The change in pH causes chnage in shape of active site that makes the inhibition of reactant to fit and bind to active sites. Therfore activity of enzyme reduces and rate of reaction also slows down. Also, changes in the pH causes protonation pattern and causes protein denaturation.