Question

describe how to measure hemoglobin in red blood cells

Answer 1

Various methods are used to measure hemoglobin in RBCs , those are

COLORIMETRIC METHODS - 1.Visual methods a.sahlis acid hematin method

b.tallqvist hemoglobin chart c.WHO hemoglobin color scale d.oxyhemoglobin method e.specific gravity method

2.Photoelectric methods - a.cyanhemoglobin method b.oxyhemoglobin method c.alkaline hematin method

GASOMETRIC METHOD

CHEMICAL METHOD

sahlis method or acid hematin method - Hemoglobin is converted to aacid hematin by N/10 HCL, the resulting brown color is compared with standard brown glass reference blocks. The intensity of the brown color depends upon on the amount of acid hematin produced, which in turn depends on the amount of hemoglobin in blood sample.

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