Question

Describe the benefits and reasons for using each type of graph.

a) Lineweaver Burk

b) Eadie Hofstee

c) Hanes_Woolf  

d) Direct

Answer 1

Lineweaver Burk:- The biggest advantage of Lineweaver Burk graph is that it gives more accurate information about inhibition.

This graph is mostly used to determine important terms of enzyme kinetics.

Eadie Hofstee:- This graph is useful in order to determine the Vmax and Km values for the enzymes.This graph is more robust against error prone data.

Hanes_Woolf :- In this graph reaction velocity is plotted as a function of velocity vs substrate concentration. Major advantage of this graph is that its accuracy. It is most accurate among other 3 graphs.

Direct:- Direct graph is used for analysing the results of kinetic experiments in which the Michaelis-Menten equation is obeyed.